All-Star Game — July 13, 1965
AL Roster
28 players
NL Roster
25 players
| Pos | Player | Team | Played |
|---|---|---|---|
| P | Juan Marichal HOF | San Francisco Giants | Yes |
| C | Joe Torre HOF | Milwaukee Braves | Yes |
| 1B | Ernie Banks HOF | Chicago Cubs | Yes |
| 2B | Pete Rose | Cincinnati Reds | Yes |
| 3B | Dick Allen HOF | Philadelphia Phillies | Yes |
| SS | Maury Wills | Los Angeles Dodgers | Yes |
| LF | Willie Stargell HOF | Pittsburgh Pirates | Yes |
| CF | Willie Mays HOF | San Francisco Giants | Yes |
| RF | Hank Aaron HOF | Milwaukee Braves | Yes |
| — | Billy Williams HOF | Chicago Cubs | Yes |
| — | Bob Gibson HOF | St. Louis Cardinals | Yes |
| — | Bob Veale | Pittsburgh Pirates | — |
| — | Cookie Rojas | Philadelphia Phillies | Yes |
| — | Don Drysdale HOF | Los Angeles Dodgers | Yes |
| — | Ed Kranepool | New York Mets | — |
| — | Frank Robinson HOF | Cincinnati Reds | Yes |
| — | Jim Maloney | Cincinnati Reds | Yes |
| — | Johnny Callison | Philadelphia Phillies | — |
| — | Johnny Edwards | Cincinnati Reds | — |
| — | Leo Cardenas | Cincinnati Reds | Yes |
| — | Roberto Clemente HOF | Pittsburgh Pirates | Yes |
| — | Ron Santo HOF | Chicago Cubs | Yes |
| — | Sammy Ellis | Cincinnati Reds | — |
| — | Sandy Koufax HOF | Los Angeles Dodgers | Yes |
| — | Turk Farrell | Houston Astros | Yes |